A secondary ‘nested PCR’ generally produces a single, major PCR product that can be sequenced to determine the integration site.The kit provides all the components and controls needed to isolate and PCR amplify the lentiviral integration sites for three cell clones, including: Multiple bands can result from multiple integrations sites, and each can be purified and sequenced separately. NucleoSpin Tissue kit to purify high-quality genomic DNA from transduced mammalian cells.Three blunt-cutter restriction enzymes, ligase, GenomeWalker adaptor, and PCR primers.Advantage 2 PCR kit for long-distance amplification from genomic DNA.NucleoSpin Gel and PCR Clean-Up kit for isolating the amplified band(s).Provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).Main Difference – Cloning Vector vs Expression VectorĬloning vector and expression vector are two types of vectors, used in recombinant DNA technology to carry foreign DNA segments into a target cell. Both cloning and expression vectors comprise of the origin of replication, unique restriction sites, and selectable marker gene in their vector sequences. Both cloning and expression vectors are self-replicative due to the presence of an origin of replication. Cloning vectors can be either plasmids, cosmids or bacteriophages. The main difference between cloning vector and expression vector is that cloning vector is used to carry foreign DNA segments into a host cell, whereas expression vector is a type of a cloning vector, which contains suitable expression signals with maximal gene expression. What are the differences between Cloning Vector and Expression Vector What are the similarities between Cloning Vector and Expression VectorĤ.
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